A STUDY OF SALIVA AND CHEEK CELLS DNA EXTRACTION WITH THE USE OF HOUSEHOLD CHEMICALS
ABSTRACT: This study was carry out to examine saliva and cheek cells DNA extraction with the use of household chemicals. This study adopts the experimental research design where data collected are tested. Quantitative assessment was performed by measuring optical density at 260/280 ratio using Nanodrop (IMPLEN NanoPhotometer®, Germany). Qualitative assessment was performed by gel electrophoresis at 0.7% agarose gel. Then 1 µL sample was mixed with 1 µL of DNA gel loading dye 6× (ThermoFisher Scientific). And 0.3 µL of SYBR safe DNA gel stain (ThermoFisher Scientific) was used as a visualizing dye. It takes approximately 10-15 min for the completion of extraction procedure. Results have indicated the good quality of DNA by gel electrophoresis and good yield with purity of 1.7-1.8 optical density. Thus, this method serves as a simple, convenient, easy-to-handle, time-saving, cheap and less laborious way for the extraction of DNA in daily routine practices with a good precision of quality and quantity that could be used for diagnostic purposes.